Product Info Summary
SKU: | M00026-2 |
---|---|
Size: | 100μl/vial |
Reactive Species: | All species |
Host: | Mouse |
Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-Myc Tag Antibody (monoclonal, 10D11)
SKU/Catalog Number
M00026-2
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-Myc Tag Antibody (monoclonal, 10D11) catalog # M00026-2. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myc Tag Antibody (monoclonal, 10D11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00026-2)
Host
Mouse
Contents
0.5mg/ml in PBS, pH7.4, containing 50% glycerol.
Clonality
Monoclonal
Clone Number
10D11
Isotype
IgG1
Immunogen
This antibody is produced by immunizing animals with a synthetic Myc tag peptide corresponding to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00026-2 is reactive to AKT1 in All species
Applications
M00026-2 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
55.686kDa
Background of Akt1
A Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology, The myc tag antibody can be used in detecting the recombinant protein with a myc tag for many purposes, for example cellular localization studies by immunofluorescence or detection by Western blotting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
1:500-1:5000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different concentration of Myc (10D11) Monoclonal Antibody
Antigen: Extracts from E.coli expressing Myc-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, 1PBS, 4 overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1TBS, 0.1% Tween-20, RT, 30mins
Protein Target Info & Infographic
Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)
Gene Name
AKT1
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55.686kDa
Superfamily
protein kinase superfamily
Alternative Names
AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1, check out the AKT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myc Tag Antibody (monoclonal, 10D11) (M00026-2)
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No publications found for M00026-2
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